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Yu Hongquan,Zhang Yan,Yu Jun
(Department of Ophthalmology,The First Teaching Hospital of
Norman Bethune University of Medical Sciences,Changchun 130021)

¡²Abstract ¡³ Objective:To observe the living cells on the surface of intraocular lenses andto compare the cellular numbers and morphology of prestaining to that ofpoststaining.Methods:Twenty adult pigment rabbit eyes were given posterior chamberintraocular lens implantation.Lenses were extracted and put into 1640 cell cultural liquidimmediately on the 1 st,3 rd,7 th,14 th and 28 th day respectively after operatrion.Thesamples were observed under inverted phase contrast microscope subsequently.Results:Wefound cells deposited on the surface of lenses from 1 st to 28 th day afteroperation.These cells contracted and lost three-demensional appearance while its numberwas less than that of prestaining obviously.Conclusion:A part of cells remove from thesurface of lenses during staining;however,we could count the number but could not affirmcellular types which would make error in cellular classification and counting.The factsuggests that we should discover new accurate and reliable methods to avoid mistakesduring experiment.
¡² Key words ¡³ intraocularlens;living cell
¡²CLC number ¡³ R-332 ¡²Documentcode ¡³ A
¡²Article ID ¡³ 0253-3707(2000)02-0142-02

1 Materials and methods

Lenses were removed from 20 adultpigment rabbit eyes which were given intraocular lens implantation on the 1 st,3 rd,7th,14 th and 28 th day after implantation.Samples were divided into five groups accordingto the different periods after operation.Each group had four lenses.We carried outcircular keratectomy first,then extracted IOLs in order to avoid the cells falling awayand scattering unevenly owing to lens ground cornea.Samples were rinsed with 0.9% sodiumchloride and put into 1640 cell cultural liquid immediately,then we observed underinverted phase contrast microscope.At last,lenses were fixed in 10% formalin and stainedaccording to Wolter^¡²1¡³ .

2 Results

There were many kinds of cells deposited onthe surface of IOL from 1 st to 28 th day after operation.Macrophages and epithelioidswere globose;fibroblast-like cells were bad spindle-shaped or multiangle-shaped.There weremany pseudopod on their surface and many melanin granules and vacuole in theircytoplasm.Unstained living cells scattered on the surface of lenses with three-dimensionaleffect.
But it was very difficult to take photograph under microscope because the IOL was aconvex lens.We could focus on only single plane.So the outlines of cells in the prestainedpictures was not as clear as poststained ones.The prestained results were compared withpoststained ones at the same position of the same sample.The cell volumes contracted andthe number reduced obviously.

3 Discussion

Since 1982,Wolter affirmed cell type with lensimplant cytology technique,the study techniques and methods have been developedcontinuously.With the development and use of electron microscope,study on cellularreaction can step into subcellular era^¡²2,3¡³ .In 1990 s,a new biological tool-specular microscope has been introduced^¡²4,5¡³ .It could observe not onlyanimal but also postoperative patient without any injury.However,this method also has itsown limits to classify cell type.
We used inverted phase contrast light microscope to observe living cells in 1640cultural liquid and compare the results of presenting with that of poststaining.Ourfinding is that the cells on the surface of IOL lost its stereoscopic outlines due todehydration;the membrane of cell shrunk with the volume contracted;the number of cellreduced obviously.It indicated that some cells fell away from IOLs during the samplepreparation.We could count the removed cell number but could not determine cellulartype.So we did not classify and count cell in our experiment.In 1991,Takahashi and hercolleagues^¡²6¡³ in Japan used specular microscope,inverted phase contrastmicroscope and scanning electron microscope to observe the cellular reaction on thesurface of IOL by three steps.Compared the specular microscope picture to extractedIOL,they found some cells fell off from the surface of IOL.
We had carried out circular keratectomy before IOL was extracted in order to avoidlens ground cornea during operation.But there were cells which fell away due tostaining.We consider that we should find new accurate and reliable methods to avoidpossible errors in experiment so that we could study the cellular reaction better.

¡²Received date¡³ 1999-07-30